Applications
FILM Core Facility Microscope
Dr. Martin Spitaler, Professor Tony Magee- Imperial College London
Situated in the Sir Alexander Fleming Building South Kensington, this system has been designed to meet a wide range of imaging requirements. Users are looking at everything from bacteria, mamalian celles, through to whole organism imaging, such as Zebra Fish. Ease of use and the technical ability of our staff was a prime consideration in the selection process.
The system also boasts an ASI XY stage with Piezo Insert, and can acquire up to 15 Z sections per second!
Future studies will include Calcium imaging and FRET.
News!! On July 4th we will be attending the official opening of this prestigious facility.
System Configuration
SPCI Image Capture Software
DIA (Dynamic Intensity Analysis) Ratiometric Software
Hamamatsu 9100-02 Camera
ASI XY Stage Piezo Z-Scanner
Live Cell 2.0 Environmental Chamber
Deconvolution Software
Professor Claudio Stern FRS.
University College London.
Chick embryo development.
Our systems are used to study Chick Embryo Development.
Professor Harry Moore
University of Sheffield- Centre for Stem Cell Biology
Multiple site time lapse allows many experiment to be run in parallel, Stem Cells Studies.
Professor Chris Marshall FRS.
Chester Beatty Laboratories London.
Several Digital Pixel systems are used in this laboratory.
They are all configured for long term lapse studies with environmental chambers and motorised stages Cancer Studies.
Professor Adrian Harwood.
University of Cardiff.
The group investigates phospho-inositide singaling and the functions of the protein kinase GSK-3.
Research in my group combines a range of cell biological, biochemical and molecular genetic
techniques in mouse and Dictyostelium model systems.
Multiple Site time lapse microscopy is used extensively to study these processes.
We are also studying neurological process, and use an environmental chamber to maintain cell viability.
Dr Samuel J. Fountain
BBSCR David Phillips Fellow
Institute of Membrane & Systems Biology
University of Leeds
Amoeba on the move. We are using time-lapse DIC imaging techniques to study
the genetically tractable model system Dictyostelium discoideum.
More information click here.
Long term imaging of cell division in mammalian cells.
Dr. Sally Wheatley- University of Sussex
Her laboratory is interested in cell division and the role of various proteins in mitosis and cytokinesis.
Typically they use cultured human cells (HeLa, U2OS and Hct116) and a combination of green
fluorescent and red fluorescent tagged cDNA constructs to localise our proteins of interest during
cell division. Experiments typicall record Multiple Position Time Lapse data over 3-7 days using our system.
System Configuration
SPCI Image Capture Software
Hamamatsu ORCA Camera
Leica DMIRB microscope
Live Cell 2.0 Environmental Chamber
Prior Motorised Stage and Illumination Control
High Speed Imaging of Platelets
Dr. Richard Farndale- University of Cambridge
Dr. Farndale is imaging fluorecently labelled platelets, and is studying ways of combating thrombus formation. Digital Pixel recommended the use of a high speed Hamamatsu 9100-02 camera system, combined with an Optical Beamplitter and PIFOC Z-Scanner. This system will allow the acquisiton of 2 wavelengths in Real Time at the frame rate of the camera- in excess of 25fps
System Configuration
SPCI Image Capture Software
DIA (Dynamic Intensity Analysis) Ratiometric Software
Hamamatsu 9100-02 Camera
PIFOC Piezo Z-Scanner
Protein-Protein Interactions in Rhodobacter sphaeroides
Armitage Group University of Oxford
Localisation of chemotaxis proteins in the bacterium Rhodobacter sphaeroides...More
