Camera Based Confocal Imaging

Infinity-SmallDigital Pixel, Compix, Visitech and Hamamatsu have joined forces to bring you the
very best in Camera based Confocal Imaging.

The VT-Infinity uniquely uses VisiTech International’s patented 2D-array laser confocal scanner technology.
The technology uses matched micro-lens and pinhole arrays to achieve optimum confocal performance, and
a single double sided galvanometer mirror to scan the sample, de-scan the fluorescent light returned from the
sample and re-scan that fluorescent light into the camera to create confocal images in real-time.

 

 


Live-Cell-SmallComputer Controlled Precise Timing

The galvanometer mirror is a very precisely controllable element and provides a pulse on each scan cycle that perfectly synchronises the camera exposure to the same point in every scan. The VT-Infinity uses the same micro-lenses and pinholes to capture each image. On the other hand spinning disc technologies, at fast capture rates capture each image using a different sector from the disk. Manufacturing tolerances in the manufacture of the disk therefore contribute  to variations in signal intensities. The VT-Infinity thus eliminates a major cause of image to image variation present in spinning disc technologies.

 

Precise integration of the 9100-series camera with the VT-Infinity AOTF also offers a unique solution for the elimination of image ‘streaking’ that may occur when using very short exposure times with frame transfer cameras.

Minimising of Photobleaching

The precise timing inherent in the Infinity also allows the system to turn off laser illumination within a few microseconds when image capture is not taking place using AOTF technology.

Variable Pinhole Better Confocality/Performance

A range of seven pinhole diameters, from 10um to 64um, is available in the VT-Infinity. The pinhole diameter is selected by software control and provides the user with the ability to match their experiment requirements in terms of microscope objective magnification, required spatial resolution, confocal depth and light throughput.


Breaking News!!    Real-Time Dual Camera Readout Now Available

In reponse to customer requirements Compix and Digital Pixel are now able to offer Real Time Dual Camera Readout.
For example using two Hamamatsu 9100-13's in dual configuration it is possible to capture 2 wavelengths at close to 30 fps, the speed that a single camera runs at.



This system is suited to dynamic imaging processes including FRET, customers who are studying cell or protein migration or those studying developmental biology. We are running a series of workshops in the near future. Call us on 01273 502176 for details